Journal: Biomedical Reports

Article Title: Effects of aged garlic extract on experimental periodontitis in mice

doi: 10.3892/br.2025.1975

Figure Lengend Snippet: Histological analysis of maxillae and surrounding periodontal tissues in mice with ligature-induced experimental periodontitis. (A and B) Hematoxylin and eosin staining. The enlarged images within the red circles in A are shown in B. (C and D) TRAP staining. The enlarged images within the red circles in C are shown in D. Representative sections for each condition (control group: n=5, AGE group: n=6) are shown using serial sections. Red arrow: osteoclasts. Scale bar, 100 µm. (E) The mean and standard deviations of the relative numbers of TRAP-positive cells on the un-ligated and ligated sides of each mouse. After analysis using one-way ANOVA followed by Tukey's multiple-comparison test, a two-tailed Student's t-test was used for two-group comparisons. * P<0.05. TRAP, tartrate-resistant acid phosphatase; AGE, aged garlic extract.

Article Snippet: Osteoclasts were visualized using a tartrate-resistant acid phosphatase (TRAP) staining kit (FUJIFILM Wako Pure Chemical Corporation).

Techniques: Staining, Control, Comparison, Two Tailed Test

Journal: Gerontology

Article Title: Nothobranchius furzeri, the Turquoise Killifish: A Model of Age-Related Osteoporosis?

doi: 10.1159/000524300

Figure Lengend Snippet: a 15-week-old N. furzeri (top: male, bottom: female). The female is smaller and displays less coloration in contrast to the male one. b Two male N. furzeri , 15 weeks old with vertebral deformities.

Article Snippet: To visualize the present osteoclasts in the vertebral area of N. furzeri , tartrate-resistant acid phosphatase staining was performed (Sigma-Aldrich, Serva).

Techniques:

Journal: Gerontology

Article Title: Nothobranchius furzeri, the Turquoise Killifish: A Model of Age-Related Osteoporosis?

doi: 10.1159/000524300

Figure Lengend Snippet: a HE-stained vertebral body of a 10-week-old male N. furzeri , original magnification, ×200. b HE-stained vertebral body of a 10-week-old male N. furzeri , original magnification, ×400, intervertebral ligament (rectangle), sagittal section. c Von Kossa/Toluidine blue-stained vertebral bodies of 8-week-old male N. furzeri , original magnification, ×400, osteoid (black arrows), bone (brown/black), ligament (bright blue), osteoblasts (blue, white arrows). d Von Kossa/toluidine blue-stained vertebral bodies of an 8-week-old male N. furzeri , original magnification, ×200. Bone tissue (brown to black), trabeculae (arrows), and sagittal section. e TRAP and toluidine blue-stained vertebral body of 12-week-old female N. furzeri , original magnification, ×200, TRAP-positive osteoclasts are indicated by arrows. TRAP, tartrate-resistant acid phosphatase.

Article Snippet: To visualize the present osteoclasts in the vertebral area of N. furzeri , tartrate-resistant acid phosphatase staining was performed (Sigma-Aldrich, Serva).

Techniques: Staining

Journal: Gerontology

Article Title: Nothobranchius furzeri, the Turquoise Killifish: A Model of Age-Related Osteoporosis?

doi: 10.1159/000524300

Figure Lengend Snippet: a Toluidine blue-stained jaw of a 12-week-old female N. furzeri , original magnification, ×100. b Toluidine blue-stained jaw of a 12-week-old female N. furzeri , original magnification, ×200, teeth (black arrows). c Toluidine blue-stained ribs of a 12-week-old male N. furzeri , original magnification, ×100. d Toluidine blue-stained rib of a 12-week-old male N. furzeri , original magnification, ×400.

Article Snippet: To visualize the present osteoclasts in the vertebral area of N. furzeri , tartrate-resistant acid phosphatase staining was performed (Sigma-Aldrich, Serva).

Techniques: Staining

Journal: Gerontology

Article Title: Nothobranchius furzeri, the Turquoise Killifish: A Model of Age-Related Osteoporosis?

doi: 10.1159/000524300

Figure Lengend Snippet: Comparison of ( a ) Ct. Th (*** p < 0.0001), ( b ) density of BV (*** p = 0.0001), and ( c ) height of vertebrae in precaudal versus caudal vertebrae within individual male and female N. furzeri (male 20 weeks of age n = 3, female 20 weeks of age n = 3, male 15 weeks of age n = 3, female 15 weeks of age n = 3, male 10 weeks of age n = 3).

Article Snippet: To visualize the present osteoclasts in the vertebral area of N. furzeri , tartrate-resistant acid phosphatase staining was performed (Sigma-Aldrich, Serva).

Techniques: Comparison

Journal: Gerontology

Article Title: Nothobranchius furzeri, the Turquoise Killifish: A Model of Age-Related Osteoporosis?

doi: 10.1159/000524300

Figure Lengend Snippet: a Ct. Th, ( b ) density of BV, and ( c ) height of vertebral bodies of 15- and 20-week-old male and female N. furzeri (male 15 weeks of age n = 3, male 20 weeks of age n = 3, female 15 weeks of age n = 3, and female 20 weeks of age n = 3). Age-related and sex-related differences were assessed with Bonferroni post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: To visualize the present osteoclasts in the vertebral area of N. furzeri , tartrate-resistant acid phosphatase staining was performed (Sigma-Aldrich, Serva).

Techniques:

Journal: Gerontology

Article Title: Nothobranchius furzeri, the Turquoise Killifish: A Model of Age-Related Osteoporosis?

doi: 10.1159/000524300

Figure Lengend Snippet: Relative ( a ) Ct. Th, ( b ) density of bone volume, and ( c ) height of vertebral bodies (in relation to body weight) of 15- and 20-week-old male and female N. furzeri (male 15 weeks of age n = 3, male 20 weeks of age n = 3, female 15 weeks of age n = 3, female 20 weeks of age n = 3). Age-related and sex-related differences were assessed with Bonferroni post hoc test. ** p < 0.01, *** p < 0.001.

Article Snippet: To visualize the present osteoclasts in the vertebral area of N. furzeri , tartrate-resistant acid phosphatase staining was performed (Sigma-Aldrich, Serva).

Techniques:

Journal: Gerontology

Article Title: Nothobranchius furzeri, the Turquoise Killifish: A Model of Age-Related Osteoporosis?

doi: 10.1159/000524300

Figure Lengend Snippet: Density of BV in N. furzeri with and without vertebral deformities. a 15-week-old males: healthy and with vertebral deformities. b 15-week-old females: healthy and with vertebral deformities.

Article Snippet: To visualize the present osteoclasts in the vertebral area of N. furzeri , tartrate-resistant acid phosphatase staining was performed (Sigma-Aldrich, Serva).

Techniques:

Journal: Gerontology

Article Title: Nothobranchius furzeri, the Turquoise Killifish: A Model of Age-Related Osteoporosis?

doi: 10.1159/000524300

Figure Lengend Snippet: a , b N.Ob/B.Pm and OV/BV in vertebrae of 8- (male n = 3, female n = 3), 12- (male n = 3, female n = 3), and 18-week (male n = 3, female n = 3)-old male and female N. furzeri . Age-related and sex-related differences were assessed with Bonferroni post hoc test. * p < 0.05.

Article Snippet: To visualize the present osteoclasts in the vertebral area of N. furzeri , tartrate-resistant acid phosphatase staining was performed (Sigma-Aldrich, Serva).

Techniques:

Journal: Gerontology

Article Title: Nothobranchius furzeri, the Turquoise Killifish: A Model of Age-Related Osteoporosis?

doi: 10.1159/000524300

Figure Lengend Snippet: a Mineral to matrix ratio (v 2 PO 4 /amide III), ( b ) proteoglycan in relation to organic matrix (GAG/amide III), ( c ) mineral maturity/crystallinity, and ( d ) cartilages proteoglycans in relation to organic matrix (GAG/amide III) in vertebrae of 5- (female n = 3, male n = 3), 10- (female n = 3, male n = 3), and 20–week (female n = 3, male n = 3)-old male and female N. furzeri . Age-related and sex-related differences were assessed by Bonferroni post hoc test. ** p < 0.01, * p < 0.05, (f) = significant differences in females, (m) = significant differences in males.

Article Snippet: To visualize the present osteoclasts in the vertebral area of N. furzeri , tartrate-resistant acid phosphatase staining was performed (Sigma-Aldrich, Serva).

Techniques:

Journal: iScience

Article Title: A novel macrolide–Del-1 axis to regenerate bone in old age

doi: 10.1016/j.isci.2024.108798

Figure Lengend Snippet:

Article Snippet: Tartrate-resistant acid phosphatase staining kit , Cosmo Bio , Cat# PMC-AK04F-COS.

Techniques: Recombinant, Construct, Transfection, Staining, Chromatin Immunoprecipitation, Magnetic Beads, Luciferase, SYBR Green Assay, Generated, Binding Assay, Gene Expression, Plasmid Preparation, Expressing, Software, Imaging, Microscopy, Fluorescence, Real-time Polymerase Chain Reaction